immunological analysis of aerobic bioreactor bovine theileriosis vaccine.

background: in this study, the pilot production of aerobic bioreactor tropical theileriosis vaccine was optimized with the aim of immunological assays for further mass production. methods: we have shown earlier the delayed type hypersensitivity (dth) assay could be used for evaluating the immunity and memory cells against specific theileria antigen in vaccinated animals. in addition, tnf-α is the principle cytokine in modulating the cytotoxic activity of cytotoxic t-lymphocytes (ctl). immunological analysis of the vaccine was performed by using two cell mediated immunity(cmi) in vitro and in vivo dth test (theilerin) and tnf-α assay. results: the results of immune responses of susceptible immunized cattle by bioreactor vaccine in comparison with conventional flask vaccine revealed a significant stimulation of immune cells by transcription of high level of tnf-α and positive reaction against theileria antigen in theilerin skin test (dth). conclusion: the equal immunological results achieved in both above mentioned vaccines verified the satisfactory immunity for aerobic bioreactor theileriosis vaccine for advance mass vaccination in the field on a large scale